China Hongx Potential Three Black Crows Candlestick Charting Pattern

After the hanging man confirmation with a down bar candlestick another down bar appeared today. If there is another down bar tomorrow a three black crows candlestick charting pattern will be formed. Price has closed below the red bold neckline support and now rest at the lower uptrend support line of the giant raising wedge formation. Next support are the overlapping 20 days and 50 days EMA support lines. If these support lines does not hold a test of 57 cents to 58 cents support band is expected. Conversely, if the unexpected happens a rebound from the lower uptrend support of the huge raising wedge will retest the 200 days EMA resistance line.

Beyond Candlesticks: New Japanese Charting Techniques Revealed (Wiley Finance)

From the "Father of Candlesticks"—penetrating new Japanese techniques for forecasting and tracking market prices and improving market timing Steve Nison has done it again. The man who revolutionized technical analysis by introducing Japanese candlestick charting techniques to Western traders is back—this time with a quartet of powerful Japanese techniques never before published or used in the West. Stunningly effective on their own, these new techniques pack an even greater wallop when teamed up with traditional trading, investing, or hedging strategies, and Steve Nison shows you how to do it. Beyond Candlesticks provides step-by-step instructions, detailed charts and graphs, and clear-cut guidance on tracking and analyzing results—everything you need to pick up these sharp new tools and take your place at the cutting edge of technical analysis. Critical praise for Steve Nison’s first book … "… destined to become the classic reference on the subject." —Charles Lebeau and David Lucas Technical Trader’s Bulletin "I believe Steve Nison’s new candlestick book is destined to become one of the truly great books for this time period.… Whether you trade futures, commodities, or equities, day trade or hold positions overnight, this book is a must." —Lee Siegfried Investor’s Library, Data Broadcasting Corp. "It is hard to be too effusive about the quality of NiSon’s work … this is clearly one of the best investment books ever written in terms of covering a subject with pedagogical ability and writing skill. The organization is impeccable … reading it was a pleasure." —Commodity Traders Consumer Report

The publisher, John Wiley & Sons
Known internationally as ``The Father of Candlesticks,'' Nison reveals more Japanese charting methods which have never been published or used in the Western world. Describes kagi, renko and three-line break charts. Provides a brief review of candlesticks and previously unavailable candlestick patterns that can be used in equities, fixed-income, foreign exchange and overseas markets.

http://www.amazon.com/Beyond-Candlesticks-Japanese-Charting-Techniques/dp/047100720X/ref=pd_sim_b_img_1

DNA Base modifications

The expression of genes is influenced by the chromatin structure of a chromosome and regions of that have low or no gene expression usually contain high levels of methylation of cytosine bases. For example, cytosine methylation, producing 5-methylcytosine, is important for X-chromosome inactivation.^[38] The average level of methylation varies between organisms, with Caenorhabditis elegans lacking cytosine methylation, while vertebrates show higher levels, with up to 1% of their DNA containing 5-methylcytosine.^[39] Despite the biological role of 5-methylcytosine it can deaminate to leave a thymine base, methylated cytosines are therefore particularly prone to mutations.^[40] Other base modifications include adenine methylation in bacteria and the glycosylation of uracil to produce the "J-base" in kinetoplastids.

cytosine	5-methylcytosine	thymine

Structure of cytosine with and without the 5-methyl group. After deamination the 5-methylcytosine has the same structure as thymine

http://en.wikipedia.org/wiki/DNA

Making the RNA copy of DNA

RNA molecules are very similar to DNA and are attracted to the unzipped DNA. The RNA molecules partner with the single stranded DNA molecules to form a mirror image copy of the DNA. RNA differs slightly from DNA: in place of every molecule of T (thymine) you have a molecule of U (uracil). So, every A in the DNA binds to a U in the newly formed RNA.

http://www.affymetrix.com/corporate/media/genechip_essentials/dna_review/Making_the_RNA_copy_of_DNA.affx

Designing a Microarray Experiment: The Basic Steps

One might ask, how does a scientist extract information about a disease condition from a dime-sized glass or silicon chip containing thousands of individual gene sequences? The whole process is based on hybridization probing, a technique that uses fluorescently labeled nucleic acid molecules as "mobile probes" to identify complementary molecules, sequences that are able to base-pair with one another. Each single-stranded DNA fragment is made up of four different nucleotides, adenine (A), thymine (T), guanine (G), and cytosine (C), that are linked end to end. Adenine is the complement of, or will always pair with, thymine, and guanine is the complement of cytosine. Therefore, the complementary sequence to G-T-C-C-T-A will be C-A-G-G-A-T. When two complementary sequences find each other, such as the immobilized target DNA and the mobile probe DNA, cDNA, or mRNA, they will lock together, or hybridize.

Now, consider two cells: cell type 1, a healthy cell, and cell type 2, a diseased cell. Both contain an identical set of four genes, A, B, C, and D. Scientists are interested in determining the expression profile of these four genes in the two cell types. To do this, scientists isolate mRNA from each cell type and use this mRNA as templates to generate cDNA with a "fluorescent tag" attached. Different tags (red and green) are used so that the samples can be differentiated in subsequent steps. The two labeled samples are then mixed and incubated with a microarray containing the immobilized genes A, B, C, and D. The labeled molecules bind to the sites on the array corresponding to the genes expressed in each cell.

http://www.ncbi.nlm.nih.gov/About/primer/microarrays.html

Can genes be turned on and off in cells?

Each cell expresses, or turns on, only a fraction of its genes. The rest of the genes are repressed, or turned off. The process of turning genes on and off is known as gene regulation. Gene regulation is an important part of normal development. Genes are turned on and off in different patterns during development to make a brain cell look and act different from a liver cell or a muscle cell, for example. Gene regulation also allows cells to react quickly to changes in their environments. Although we know that the regulation of genes is critical for life, this complex process is not yet fully understood.

Gene regulation can occur at any point during gene expression, but most commonly occurs at the level of transcription (when the information in a gene’s DNA is transferred to mRNA). Signals from the environment or from other cells activate proteins called transcription factors. These proteins bind to regulatory regions of a gene and increase or decrease the level of transcription. By controlling the level of transcription, this process can determine the amount of protein product that is made by a gene at any given time.

http://ghr.nlm.nih.gov/handbook/howgeneswork/geneonoff