Although each cell contains a full complement of DNA, cells use genes selectively. Some genes enable cells to make proteins needed for basic functions; dubbed housekeeping genes, they are active in many types of cells. Other genes, however, are inactive most of the time. Some genes play a role in early development of the embryo and are then shut down forever. Many genes encode proteins that are unique to a particular kind of cell and that give the cell its character - making a brain cell, say, different from a bone cell. A normal cell activates just the genes it needs at the moment and actively suppresses the rest.
Nucleases are enzymes that cut DNA strands by catalyzing the hydrolysis of the phosphodiester bonds. Nucleases that hydrolyse nucleotides from the ends of DNA strands are called exonucleases, while endonucleases cut within strands. The most frequently-used nucleases in molecular biology are the restriction endonucleases, which cut DNA at specific sequences. For instance, the EcoRV enzyme shown to the left recognizes the 6-base sequence 5′-GAT|ATC-3′ and makes a cut at the vertical line. In nature, these enzymes protect bacteria against phage infection by digesting the phage DNA when it enters the bacterial cell, acting as part of the restriction modification system.[78] In technology, these sequence-specific nucleases are used in molecular cloning and DNA fingerprinting.
Enzymes called DNA ligases can rejoin cut or broken DNA strands.[79] Ligases are particularly important in lagging strand DNA replication, as they join together the short segments of DNA produced at the replication fork into a complete copy of the DNA template. They are also used in DNA repair and genetic recombination.
